What method could we use to sequence the mRNA of the plant and quantify it at the same time

A real time PCR assay for the peanut allergen Ara h 2 has been developed with a sensitivity of 10 copies per reaction of the target gene [51];

From: Food Toxicants Analysis, 2007

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Page 2

	Replicates per group
	3	5	10
Effect size (fold change)
1.25	17 %	25 %	44 %
1.5	43 %	64 %	91 %
2	87 %	98 %	100 %
Sequencing depth (millions of reads)
3	19 %	29 %	52 %
10	33 %	51 %	80 %
15	38 %	57 %	85 %

Example of calculations for the probability of detecting differential expression in a single test at a significance level of 5 %, for a two-group comparison using a Negative Binomial model, as computed by the RNASeqPower package of Hart et al. [190]. For a fixed within-group variance (package default value), the statistical power increases with the difference between the two groups (effect size), the sequencing depth, and the number of replicates per group. This table shows the statistical power for a gene with 70 aligned reads, which was the median coverage for a protein-coding gene for one whole-blood RNA-seq sample with 30 million aligned reads from the GTEx Project [214]